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50 Similarly, upregulation of thymidylate synthase (TYMS), a target of 5-fluorouracil (5-FU), contributes to resistance in colorectal cancer by augmenting DNA repair pathways

Because nicotinamide would otherwise re-enter the NAD+ salvage pathway through NAMPT and NMN, NNMT activity sits at a junction between cellular methylation capacity and NAD+ regeneration, which is why the enzyme became a target after Kraus and colleagues (Nature, 2014) reported the consequences of antisense Nnmt knockdown in white adipose tissue and liver

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